.
Allow the dog to do some sniffing. If he does not mount the bitch, stroke his prepuce a few times until you feel that he is getting an erection. You may have to lift him in the mounting position to encourage him. Most of the time you will not have to do anything to get him to mount the bitch.
Once he mounts the bitch quickly place the collection bag over his penis and push the prepuce up and behind the bulb. The prepuce must be behind the bulb if not the dog can not become fully erect and the process will be very painful for him. Do not allow him to become erect inside of the prepuce.
You will be letting your hand mimic the “feel” of the vagina by holding his penis so that you can put slight pressure behind the bulb of the penis. It will help to make sure your hands are warm.
Once the dog begins the thrusting actions you are pretty assured of getting a collection. Continue holding the penis until you have collected the entire sperm fractions and some of the prostate fluid.
The prostate fluid is clear like water. The sperm fraction is very thick and milky white. When the two are mixed the semen will be cloudy but thinned out and watery. You must be gentle with it and not cause any bubbles because they will be very difficult to get out of your insemination rod.
Gently remove the collection bag from the penis and put the dog away in a safe place. Check to make sure his penis has retracted back into the prepuce.
Studies have shown that you will maximize the quantity of collection when using a teaser bitch.
Attach the syringe to the insemination rod. Pull the semen into the syringe by pulling the end of the rod into the semen in the collection bag. Holding the syringe and rod upward and with a light push out all the air from the syringe and rod pushing semen almost to the tip of the rod.
You may want to check to make sure the semen is alive and active by placing a single drop of semen on a slide and using a microscope.
Now you are ready for the Insemination process.
Make sure that the bitch has emptied her bladder prior to the insemination. With larger bitches you may need to have a second person to help. The best tool to use is an insemination rod. Some breeders use soft flexible catheters but you may have better luck with the rods and sometimes the soft flexible catheters may contain latex which will kill the sperm. Make sure your syringes are labeled “latex free”. After you have completed all the A.I.s , throw away the rod and syringe away. Do not use them on another bitch. You can clean between A.I.s by rinsing well in very hot water, followed by a rinse in normal saline solution and let them completely dry in a sunny window before using for a 2nd and 3rd time on the same bitch.